AICAR

Also Known As: Acadesine, AICA-riboside, 5-aminoimidazole-4-carboxamide ribonucleotide, AICAR nucleoside, ZMP precursor, Acadesine AICAR, AMPK activator AICAR, Exercise mimetic AICAR, Metabolic research AICAR, AICA ribonucleoside, Purine nucleoside analog AICAR, AMPK activator, exercise in a bottle, acadesine, ZMP

AICAR is a research-grade Purine nucleoside analog targeting AMP-activated protein kinase studied in Weight Loss & Metabolism research. It is supplied strictly for in vitro laboratory research use only - not for human or animal consumption, and not FDA-approved.

Evidence Tier: Research Compound - No approved human use; sold for laboratory research only.

An exercise-mimetic AMPK activator; banned in sport, mostly preclinical.

At A Glance

AICAR Key Research Facts
CategoryWeight Loss & Metabolism
Compound ClassPurine nucleoside analog; cell-permeable AMPK activator (converted intracellularly to ZMP)
Molecular TargetAMP-activated protein kinase (AMPK) activation via ZMP (AMP mimetic); PGC-1alpha upregulation; GLUT4 translocation; mitochondrial biogenesis
Molecular Weight258.23 Da
Amino Acid Sequencesmall molecule nucleoside
CAS Number2627-69-2
Half-Life~2 hours
WADA Statusprohibited
Evidence TierResearch Compound

Mechanism Of Action

It enters your cells and flips a master energy switch called AMPK. When this switch is flipped, the cell starts burning fat for energy and builds more stamina engines (mitochondria).

Studied For

  • AMPK / metabolic-stress research
  • Insulin-resistance models
  • Endurance research
  • Exercise mimetic
  • Fat oxidation via AMPK
  • Glucose uptake enhancement
  • Cardiac protection / ischemia
  • Obesity models
  • Metabolic syndrome research
  • WADA prohibited performance substance research
  • Mitochondrial biogenesis
  • Type 2 diabetes models
  • Cancer metabolism research
  • AMPK pathway signaling
  • Anti-inflammatory via AMPK
  • endurance enhancement
  • fat burning
  • metabolic regulator
  • ischemic protection
  • muscle metabolism

Reported Research Findings

Mostly in vitro and animal: increased glucose uptake, fat oxidation, and running endurance. Acadesine reached human cardiac-surgery trials but was never approved. No established human metabolic or performance efficacy.

Safety And Handling Notes

Transient hyperuricemia and headache in cardiac trials; broad AMPK activation can be context-dependently tumor-promoting; long-term human safety unknown.

Investigational with no safe non-clinical dose. Named on the WADA 2026 Prohibited List (S4.4.1, AMPK activators), prohibited at all times and non-Specified. Hyperuricemia and gout caution. Research use only.

Regulatory Status

Investigational / research chemical; not DEA-controlled. Named on the WADA 2026 Prohibited List under S4.4.1 (activators of the AMP-activated protein kinase); prohibited at all times and a non-Specified substance.

Full Reference Detail

Related Research Guides

Maintained By The Pep Nation Lab Research Team. See Our Editorial Standards And Research Methodology For How This Reference Is Sourced And Reviewed.

This Reference Is Provided For In Vitro Laboratory Research Use Only. Not For Human Consumption. See The Full Research Disclaimer.

References And Citations

  1. usada.org
  2. selleckchem.com
Spec Sheet

AICAR

Also Known As: Acadesine, AICA-riboside, 5-aminoimidazole-4-carboxamide ribonucleotide, AICAR nucleoside, ZMP precursor, Acadesine AICAR, AMPK activator AICAR, Exercise mimetic AICAR, Metabolic research AICAR, AICA ribonucleoside, Purine nucleoside analog AICAR, AMPK activator, exercise in a bottle, acadesine, ZMP

Research CompoundWeight Loss & Metabolism

Explain Like I'm 5

  • AICAR tricks your body into thinking it just did a massive workout, even if it was just sitting still. It turns on your fat-burning mode and makes muscles act like they have been exercising for hours.

An exercise-mimetic AMPK activator; banned in sport, mostly preclinical.

Identity & Classification
AICAR Key Research Facts
ClassPurine nucleoside analog; cell-permeable AMPK activator (converted intracellularly to ZMP)
Molecular TargetAMP-activated protein kinase (AMPK) activation via ZMP (AMP mimetic); PGC-1alpha upregulation; GLUT4 translocation; mitochondrial biogenesis
CategoryWeight Loss & Metabolism
Molecular Weight258.23 Da
Amino Acid Sequencesmall molecule nucleoside
CAS Number2627-69-2
Parent CompoundZMP precursor
Research Metrics at a Glance
2,829
PubMed Citations

Related Compounds

Research Use Only. Not For Human Or Veterinary Use. Information Provided For Laboratory Research Purposes Only.

Research Reference Only — Not Medical Advice. For In Vitro Laboratory Use Only.
Typical Dose250–500 mg
FrequencyOnce daily
Cycle On4–8 weeks
Cycle Off4 weeks
Route(s)
SubcutaneousIntravenous (research infusion at 500 mg/kg in rodents)
Research Notes

AMPK activator (5-aminoimidazole-4-carboxamide ribonucleotide). Endurance and metabolic research. Rodent research used very high doses (500 mg/kg IP) — human-equivalent subcutaneous doses far lower. WADA-prohibited substance; research-only context only.

Sequence Motif Viewer

23 Residues
SMALLMOLECULENUCLEOSIDE
BasicAcidicPolarNon-PolarAromaticSpecial
CompoundCompoundTargetReceptor / TargetIt enters your cells...Downstream PathwayResearch EffectStudied Effect

Compare AICAR

AICAR Frequently Asked Questions

What Is AICAR?

An exercise-mimetic AMPK activator; banned in sport, mostly preclinical.

How Does AICAR Work?

It enters your cells and flips a master energy switch called AMPK. When this switch is flipped, the cell starts burning fat for energy and builds more stamina engines (mitochondria).

What Has AICAR Been Studied For?

In the referenced literature, AICAR has been studied for AMPK / metabolic-stress research, Insulin-resistance models, Endurance research, Exercise mimetic, Fat oxidation via AMPK, Glucose uptake enhancement, Cardiac protection / ischemia, Obesity models, Metabolic syndrome research, WADA prohibited performance substance research, Mitochondrial biogenesis, Type 2 diabetes models, Cancer metabolism research, AMPK pathway signaling, Anti-inflammatory via AMPK, endurance enhancement, fat burning, metabolic regulator, ischemic protection, muscle metabolism. This summarizes research focus only and is not a claim of efficacy.

What Is The Reported Half-Life Of AICAR?

AICAR has a reported half-life of ~2 hours in the referenced literature.

Is AICAR Approved For Human Use?

No. AICAR is supplied strictly for in vitro laboratory research use only. It is not intended for human or animal consumption, ingestion, or injection, and it has not been evaluated by the FDA.

Regional Research Availability

Pep Nation Lab supplies research-grade AICAR to qualified researchers and scientific institutions nationwide. Browse verified availability and per-region documentation by state:

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